Protein A sepharose Purification kit
Affinity purified Protein A antibodies, developed in mouse, were conjugated to
NHS-sepharose. Protein A is a 42 kDa surface protein found in the cell wall of
Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse
IgG and others and is therefore suitable for purification and detection of immunoglobulins. Therefore, Croyez Protein A sepharose is an efficient
technique for IgG purification.
Recombinant protein A is immobilized at over 5 mg antibody per mL 50%
slurry and this kit allows a rapid and efficient affinity purification of active IgG
proteins. The affinity resin allows an efficient binding of IgG proteins without
the need for preliminary steps and calibrations. The affinity bound IgG proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.
NHS-sepharose. Protein A is a 42 kDa surface protein found in the cell wall of
Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse
IgG and others and is therefore suitable for purification and detection of immunoglobulins. Therefore, Croyez Protein A sepharose is an efficient
technique for IgG purification.
Recombinant protein A is immobilized at over 5 mg antibody per mL 50%
slurry and this kit allows a rapid and efficient affinity purification of active IgG
proteins. The affinity resin allows an efficient binding of IgG proteins without
the need for preliminary steps and calibrations. The affinity bound IgG proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.
Component:
Note: Sepharose is 1:1 suspension in Phosphate Buffered Saline, pH 7.4, containing 0.05% sodium azide as a preservative.
Product capacity:
The binding and elution capacity of 1 mL settled Protein A sepharose are commonly more than 1 mg of IgG proteins. Trying different elution buffers for optimal results is recommended.
Materials Required but Not Provided:
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice
| Reagent | Quantity for 10 rxns (C07005-K02) |
Quantity for 5 rxns (C07005-K01) |
composition |
| Protein A sepharose |
2 mL X 1 vial | 1 mL X 1 vial | 50% slurry of Protein A sepharose in 1X Phosphate Buffered Saline |
| Wash Buffer (10X concentration) |
5.0 mL X 1 vial | 2.5 mL X 1 vial | 100 mM Tris/Cl pH 7.5; 1.5 M NaCl; 5 mM EDTA |
| Elution Buffer | 10 mL X 1 vial | 5 mL X 1 vial | 0.1 M Glycine pH 2.7 |
| Neutralization Buffer | 2 mL X 1 vial | 1 mL X 1 vial | 2 M Tris pH 8.0 |
| spin column | 10 pcs | 5 pcs | |
| collection tube | 10 tubes | 5 tubes |
Product capacity:
The binding and elution capacity of 1 mL settled Protein A sepharose are commonly more than 1 mg of IgG proteins. Trying different elution buffers for optimal results is recommended.
Materials Required but Not Provided:
- Micro-centrifuge capable of 15,000 x g
- 1.5 mL Centrifuge tubes
- End-over-end rotator
- CoIP Lysis Buffer (mild reaction): 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.5% NP-40
- RIPA (vigorous reaction): 100 mM Tris/Cl pH 7.5; 300 mM NaCl; 0.2% Sodium Deoxycholate (or 0.1% SDS); 2% NP-40
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice